Characterization of North American Armillaria species by nuclear DNA content and RFLP analysis
نویسندگان
چکیده
Twenty-six isolates representing nine North American Armillaria species were investigated with flow cytometry and RFLP (restriction fragment length polymorphism) analyses to determine their nuclear DNA content and RFLP profile. Three putatively diploid isolates of A. ostoyae, A. gemina, A. calvescens, A. sinapina, A. mellea, A. gallica, A. nabsnona, and North American Biological Species (NABS) X were analyzed, and two putatively diploid isolates of NABS XI also were analyzed. Nuclear DNA contents of Armillaria species were 0.11-0.17 pg per nucleus (55-84 X 10" bp/C), depending on species. Among the nine North American Armillaria species tested, A. ostoyae, A. gemirza, and A. mellea possessed relatively small nuclear DNA contents (0.1 1-0.12 pg per nucleus), whereas A. gallica possessed a relatively large nuclear DNA content (0.17 pg per nucleus). A. nabsno,na has a slightly larger nuclear DNA content (0.13pg per nucleus) than A. ostojae, A. gemina, and A. mellea. Other species (A. calvescens, A. sinapina, NABS X, and NABS XI) possessed moderate nuclear DNA contents (ca 0.15 pg per nucleus). Polymerase chain reaction (PCR) and RFLP of the intergenic spacer region-1 (IGS-1) generated banding patterns for nine Armillaria species. In addition to previously reported banding patterns, new banding patterns are Accepted for publication Xfa1-cl1 24. 2000. This manuscript has been assigned J o ~ ~ r n a l Serie, No. 12652, Agricultural Reseal-ch Dl\-ision, C n i v e r s i ~ of Nebraska. "~irrent address: Department of Forest Resources, University of Idaho, hloscow. ID 83844 USA, email: [email protected]~~ presented for A. gemina, A. calvescens, A. melka, and A. gallica.
منابع مشابه
Short title: Kim et al: Characterization of North American Armillaria species Characterization of North American Armillaria species by DNA content and RFLP analysis
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